Selected publications

Donaldson J, Kleinjan DJ, Rosser S. Synthetic biology approaches for dynamic CHO cell engineering. Curr Opin Biotechnol. 2022 Oct 1;78:102806. doi: 10.1016/j.copbio.2022.102806. Epub ahead of print. PMID: 36194920.

Bryson JW, Auxillos JY, Rosser SJ. Multiplexed activation in mammalian cells using a split-intein CRISPR/Cas12a based synthetic transcription factor. Nucleic Acids Res. 2022 Jan 11;50(1):549-560. doi: 10.1093/nar/gkab1191. PMID: 34908140

Wang J, Zhao D, Li J, Hu M, Xin X, Price MA, Li Q, Liu L, Li S, Rosser SJ, Zhang C, Bi C, Zhang X. Helicase-AID: A novel molecular device for base editing at random genomic loci. Metab Eng. 2021 Sep;67:396-402. doi: 10.1016/j.ymben.2021.08.005. PMID: 34411701.

Sloan WT, Nnaji CF, Lunn M, Curtis TP, Colloms SD, Couto JM, Pinto AJ, Connelly S, Rosser SJ. Drift dynamics in microbial communities and the effective community size. Environ Microbiol. 2021 May;23(5):2473-2483. doi: 10.1111/1462-2920.15453. PMID: 33684262.

Donaldson JS, Dale MP, Rosser SJ. Decoupling Growth and Protein Production in CHO Cells: A Targeted Approach. Front Bioeng Biotechnol. 2021 Jun 2;9:658325. doi: 10.3389/fbioe.2021.658325. PMID: 34150726. 

Zhao D, Li J, Li S, Xin X, Hu M, Price MA, Rosser SJ, Bi C, Zhang X. Publisher Correction: Glycosylase base editors enable C-to-A and C-to-G base changes. Nat Biotechnol. 2021 Jan;39(1):115. doi: 10.1038/s41587-020-0648-3. PMID: 32728159. 

Zhao D, Li J, Li S, Xin X, Hu M, Price MA, Rosser SJ, Bi C, Zhang X. Glycosylase base editors enable C-to-A and C-to-G base changes. Nat Biotechnol. 2021 Jan;39(1):35-40. doi: 10.1038/s41587-020-0592-2. PMID: 32690970.

Zulkower V, Rosser S. DNA Chisel, a versatile sequence optimizer. Bioinformatics. 2020 Aug 15;36(16):4508-4509. doi: 10.1093/bioinformatics/btaa558. PMID: 32647895.

Zulkower V, Rosser S. DNA Features Viewer: a sequence annotation formatting and plotting library for Python. Bioinformatics. 2020 Aug 1;36(15):4350-4352. doi: 10.1093/bioinformatics/btaa213. PMID: 32637988. 

Yu S, Price MA, Wang Y, Liu Y, Guo Y, Ni X, Rosser SJ, Bi C, Wang M. CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis. ACS Synth Biol. 2020 Jul 17;9(7):1781-1789. doi: 10.1021/acssynbio.0c00151. PMID: 32551562.

Markakis K, Lowe PT, Davison-Gates L, O'Hagan D, Rosser SJ, Elfick A. An Engineered E. coli Strain for Direct in Vivo Fluorination. Chembiochem. 2020 Jul 1;21(13):1856-1860. doi: 10.1002/cbic.202000051. PMID: 32003116. 

Szymanski E, Bates T, Cachat E, Calvert J, Catts O, Nelson LJ, Rosser SJ, Smith RDJ, Zurr I. Crossing Kingdoms: How Can Art Open Up New Ways of Thinking About Science? Front Bioeng Biotechnol. 2020 Jul 3;8:715. doi: 10.3389/fbioe.2020.00715. PMID: 32733867.

Price MA, Cruz R, Bryson J, Escalettes F, Rosser SJ. Expanding and understanding the CRISPR toolbox for Bacillus subtilis with MAD7 and dMAD7. Biotechnol Bioeng. 2020 Jun;117(6):1805-1816. doi: 10.1002/bit.27312. PMID: 32077487.

Dale MP, Moses T, Johnston EJ, Rosser SJ. A systematic comparison of triterpenoid biosynthetic enzymes for the production of oleanolic acid in Saccharomyces cerevisiae. PLoS One. 2020 May 1;15(5):e0231980. doi: 10.1371/journal.pone.0231980. PMID: 32357188.

Johnston EJ, Moses T, Rosser SJ. The wide-ranging phenotypes of ergosterol biosynthesis mutants, and implications for microbial cell factories. Yeast. 2020 Jan;37(1):27-44. doi: 10.1002/yea.3452. PMID: 31800968.

Olorunniji FJ, Lawson-Williams M, McPherson AL, Paget JE, Stark WM, Rosser SJ. Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing. Nucleic Acids Res. 2019 Dec 2;47(21):11452-11460. doi: 10.1093/nar/gkz936. PMID: 31667500.

Price MA, Cruz R, Baxter S, Escalettes F, Rosser SJ. CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis. PLoS One. 2019;14(1):e0210121. doi: 10.1371/journal.pone.0210121. PMID: 30615645.

Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS. Author Correction: Building a global alliance of biofoundries. Nat Commun. 2019 Jul 11;10(1):3132. doi: 10.1038/s41467-019-10862-1. PMID: 31296848.

Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS. Building a global alliance of biofoundries. Nat Commun. 2019 May 9;10(1):2040. doi: 10.1038/s41467-019-10079-2. PMID: 31068573.

Zhao J, Pokhilko A, Ebenhöh O, Rosser SJ, Colloms SD. A single-input binary counting module based on serine integrase site-specific recombination. Nucleic Acids Res. 2019 May 21;47(9):4896-4909. doi: 10.1093/nar/gkz245. PMID: 30957849.

Chen Y, Dolt KS, Kriek M, Baker T, Downey P, Drummond NJ, Canham MA, Natalwala A, Rosser S, Kunath T. Engineering synucleinopathy-resistant human dopaminergic neurons by CRISPR-mediated deletion of the SNCA gene. Eur J Neurosci. 2019 Feb;49(4):510-524. doi: 10.1111/ejn.14286. PMID: 30472757.

Merrick CA, Zhao J, Rosser SJ. Serine Integrases: Advancing Synthetic Biology. ACS Synth Biol. 2018 Feb 16;7(2):299-310. doi: 10.1021/acssynbio.7b00308. Epub 2018 Jan 30. PMID: 29316791.

Kleinjan DA, Wardrope C, Nga Sou S, Rosser SJ. Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Nat Commun. 2017 Oct 30;8(1):1191. doi: 10.1038/s41467-017-01222-y. PMID: 29084946.

Olorunniji FJ, Merrick C, Rosser SJ, Smith MCM, Stark WM, Colloms SD. Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family. Methods Mol Biol. 2017;1642:303-323. doi: 10.1007/978-1-4939-7169-5_19. PMID: 28815508.

Olorunniji FJ, Rosser SJ, Marshall Stark W. Purification and In Vitro Characterization of Zinc Finger Recombinases. Methods Mol Biol. 2017;1642:229-245. doi: 10.1007/978-1-4939-7169-5_15. PMID: 28815504.

Olorunniji FJ, McPherson AL, Rosser SJ, Smith MCM, Colloms SD, Stark WM. Control of serine integrase recombination directionality by fusion with the directionality factor. Nucleic Acids Res. 2017 Aug 21;45(14):8635-8645. doi: 10.1093/nar/gkx567. PMID: 28666339.

Trubitsyna M, Michlewski G, Finnegan DJ, Elfick A, Rosser SJ, Richardson JM, French CE. Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection. Nucleic Acids Res. 2017 Jun 2;45(10):e89. doi: 10.1093/nar/gkx113. PubMed PMID: 28204586.

Jensen MV, Horsfall LE, Wardrope C, Togneri PD, Marles-Wright J, Rosser SJ. Characterisation of a New Family of Carboxyl Esterases with an OsmC Domain. PLoS One. 2016 Nov 16;11(11):e0166128. doi: 10.1371/journal.pone.0166128. PubMed PMID: 27851780.

Boeke JD, Church G, Hessel A, Kelley NJ, Arkin A, Cai Y, Carlson R, Chakravarti A, Cornish VW, Holt L, Isaacs FJ, Kuiken T, Lajoie M, Lessor T, Lunshof J, Maurano MT, Mitchell LA, Rine J, Rosser S, Sanjana NE, Silver PA, Valle D, Wang H, Way JC, Yang L. GENOME ENGINEERING. The Genome Project-Write. Science. 2016 Jul 8;353(6295):126-7. doi: 10.1126/science.aaf6850. PubMed PMID: 27256881.

Fletcher L, Rosser S, Elfick A. Exploring Synthetic and Systems Biology at the University of Edinburgh. Biochem Soc Trans. 2016 Jun 15;44(3):692-5. doi: 10.1042/BST20160006. Review. PubMed PMID: 27284029.

Bowyer J, Zhao J, Subsoontorn P, Wong W, Rosser S, Bates D. Mechanistic Modeling of a Rewritable Recombinase Addressable Data Module. IEEE Trans Biomed Circuits Syst. 2016 Dec;10(6):1161-1170. doi: 10.1109/TBCAS.2016.2526668. PubMed PMID: 27244749.

Yu N, Nützmann HW, MacDonald JT, Moore B, Field B, Berriri S, Trick M, Rosser SJ, Kumar SV, Freemont PS, Osbourn A. Delineation of metabolic gene clusters in plant genomes by chromatin signatures. Nucleic Acids Res. 2016 Mar 18;44(5):2255-65. doi: 10.1093/nar/gkw100. PubMed PMID: 26895889.

Olorunniji FJ, Rosser SJ, Stark WM. Site-specific recombinases: molecular machines for the Genetic Revolution.  Biochem J. 2016 Mar 15;473(6):673-84. doi: 10.1042/BJ20151112. Review. PubMed PMID: 26965385.

Kanigowska P, Shen Y, Zheng Y, Rosser S, Cai Y. Smart DNA Fabrication Using Sound Waves: Applying Acoustic Dispensing Technologies to Synthetic Biology. J Lab Autom. 2016 Feb;21(1):49-56. doi: 10.1177/2211068215593754. Epub 2015 Jul 10. PubMed PMID: 26163567; PubMed Central PMCID: PMC4814025.

Bowyer J, Jia Zhao, Rosser S, Colloms S, Bates D. Development and experimental validation of a mechanistic model of in vitro DNA recombination. Conf Proc IEEE Eng Med Biol Soc. 2015 Aug;2015:945-8. doi: 10.1109/EMBC.2015.7318519. PubMed PMID: 26736419.

Fogg PC, Colloms S, Rosser S, Stark M, Smith MC. New applications for phage integrases. J Mol Biol. 2014 Jul 29;426(15):2703-16. doi: 10.1016/j.jmb.2014.05.014. Epub 2014 May 22. Review. PubMed PMID: 24857859; PubMed Central PMCID: PMC4111918.

Kemen AC, Honkanen S, Melton RE, Findlay KC, Mugford ST, Hayashi K, Haralampidis K, Rosser SJ, Osbourn A. Investigation of triterpene synthesis and regulation in oats reveals a role for β-amyrin in determining root epidermal cell patterning. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8679-84. doi: 10.1073/pnas.1401553111. Epub 2014 May 27. PubMed PMID: 24912185; PubMed Central  PMCID: PMC4060722.

Colloms SD, Merrick CA, Olorunniji FJ, Stark WM, Smith MC, Osbourn A, Keasling JD, Rosser SJ. Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination. Nucleic Acids Res. 2014 Feb;42(4):e23. doi: 10.1093/nar/gkt1101. Epub 2013 Nov 12. PubMed PMID: 24225316; PubMed Central PMCID: PMC3936721.

Mugford ST, Louveau T, Melton R, Qi X, Bakht S, Hill L, Tsurushima T, Honkanen S, Rosser SJ, Lomonossoff GP, Osbourn A. Modularity of plant metabolic gene clusters: a trio of linked genes that are collectively required for acylation of triterpenes in oat. Plant Cell. 2013 Mar;25(3):1078-92. doi: 10.1105/tpc.113.110551. Epub 2013 Mar 26. Erratum in: Plant Cell. 2013 Sep;25(9):3632. PubMed PMID: 23532069; PubMed Central PMCID: PMC3634678.

Olorunniji FJ, Buck DE, Colloms SD, McEwan AR, Smith MC, Stark WM, Rosser SJ. Gated rotation mechanism of site-specific recombination by ϕC31 integrase. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19661-6. doi: 10.1073/pnas.1210964109. Epub 2012 Nov 12. PubMed PMID: 23150546;  PubMed Central PMCID: PMC3511744.

Osbourn AE, O'Maille PE, Rosser SJ, Lindsey K. Synthetic biology. 4th New Phytologist Workshop, Bristol, UK, June 2012. New Phytol. 2012 Nov;196(3):671-7. doi: 10.1111/j.1469-8137.2012.04374.x. PubMed PMID: 23043589.

Martino C, Kim SH, Horsfall L, Abbaspourrad A, Rosser SJ, Cooper J, Weitz DA. Protein expression, aggregation, and triggered release from polymersomes as artificial cell-like structures. Angew Chem Int Ed Engl. 2012 Jun 25;51(26):6416-20. doi: 10.1002/anie.201201443. Epub 2012 May 29. PubMed PMID: 22644870.

Chanasakulniyom M, Martino C, Paterson D, Horsfall L, Rosser S, Cooper JM. Expression of membrane-associated proteins within single emulsion cell facsimiles. Analyst. 2012 Jul 7;137(13):2939-43. doi: 10.1039/c2an35047e. Epub 2012 Mar 22. PubMed PMID: 22441306.

Martino C, Horsfall L, Chen Y, Chanasakulniyom M, Paterson D, Brunet A, Rosser S, Yuan YJ, Cooper JM. Cytoskeletal protein expression and its association within the hydrophobic membrane of artificial cell models. Chembiochem. 2012 Apr 16;13(6):792-5. doi: 10.1002/cbic.201200038. Epub 2012 Mar 12. PubMed PMID: 22411693.

Seth-Smith HM, Edwards J, Rosser SJ, Rathbone DA, Bruce NC. The explosive-degrading cytochrome P450 system is highly conserved among strains of Rhodococcus spp. Appl Environ Microbiol. 2008 Jul;74(14):4550-2. doi: 10.1128/AEM.00391-08. Epub 2008 May 16. PubMed PMID: 18487400; PubMed Central PMCID: PMC2493155.

Hannink NK, Subramanian M, Rosser SJ, Basran A, Murray JA, Shanks JV, Bruce NC. Enhanced transformation of tnt by tobacco plants expressing a bacterial nitroreductase. Int J Phytoremediation. 2007 Sep-Oct;9(5):385-401. doi: 10.1080/15226510701603916. PubMed PMID: 18246725.

Travis ER, Bruce NC, Rosser SJ. Short term exposure to elevated trinitrotoluene concentrations induced structural and functional changes in the soil bacterial community. Environ Pollut. 2008 May;153(2):432-9. Epub 2007 Nov 1. PubMed PMID: 17935846.

Travis ER, Hannink NK, Van der Gast CJ, Thompson IP, Rosser SJ, Bruce NC. Impact of transgenic tobacco on trinitrotoluene (TNT) contaminated soil community. Environ Sci Technol. 2007 Aug 15;41(16):5854-61. PubMed PMID: 17874797.

Travis ER, Bruce NC, Rosser SJ. Microbial and plant ecology of a long-term TNT-contaminated site. Environ Pollut. 2008 May;153(1):119-26. Epub 2007 Sep 6. PubMed PMID: 17825462.

Seth-Smith HM, Rosser SJ, Basran A, Travis ER, Dabbs ER, Nicklin S, Bruce NC. Cloning, sequencing, and characterization of the hexahydro-1,3,5-Trinitro-1,3,5-triazine degradation gene cluster from Rhodococcus rhodochrous. Appl Environ Microbiol. 2002 Oct;68(10):4764-71. PubMed PMID: 12324318; PubMed Central PMCID: PMC126434.

Larsen NA, Turner JM, Stevens J, Rosser SJ, Basran A, Lerner RA, Bruce NC, Wilson IA. Crystal structure of a bacterial cocaine esterase. Nat Struct Biol. 2002 Jan;9(1):17-21. PubMed PMID: 11742345.