Dr Susie Sou

Susie (Si Nga) Sou

Background


Susie obtained her BSc and MRes degrees at Imperial College London, in the area of biochemistry and biochemical research. She did her PhD study in the department of Chemical Engineering at Imperial College London, where she focused her research on bioprocess optimisation and modelling on biopharmaceutical production in Chinese hamster ovary cells. After the completion of her PhD degree, she worked briefly as a post-doctoral research associate with Dr Cleo Kontoravdi on bioprocess modelling and she joined the Rosser lab in May 2016, focusing her research on recombinant protein production and optimisation through CHO cell engineering.

Research


Susie's research in the Rosser lab is divided into three main scopes: 1. Generating site-specific stable gene integration in CHO cells to identify preferable sites within the genome for optimal rProtein production; 2. Establishing a CRISPR-dCas9 system as synthetic transcription factor to manipulate host cell endogenous gene expression; 3. Creation of an artificial CHO chromosome to aid rProtein production and genome characterisation.

Publications


Sou SN, Sellick C, Lee K, Nayyar K, Jedrzejewski PM, Polizzi KM, Kontoravdi C. (2016). Model-based investigation of intracellular processes determining antibody Fc-glycosylation under mild hypothermia (under review)

Sou SN, Sellick C, Lee K, Mason A, Kyriakopoulos S, Polizzi KM, Kontoravdi C. (2015). Understanding the impact of different bioprocess conditions on monoclonal antibody glycosylation in CHO cell culture through experimental and computational analyses. BMC Proceedings 2015, 9(Suppl 9):O6 

Sou SN, Sellick C, Lee K, Mason A, Kyriakopoulos S, Polizzi KM, Kontoravdi C. (2015). How does mild hypothermia affect monoclonal antibody glycosylation? Biotechnol. Bioeng., 112: 1165–1176.

Sou SN, Kontoravdi C, Polizzi KM. (2013). Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells. Adv Bios Biotech. 4(12): 1013 – 1019.

Jimenez del Val I, Jedrzejewski PM, Exley K, Sou SN, Kyriakopoulos S, Polizzi KM, Kontoravdi C (2012). Application of quality by design paradigm to the manufacture of protein therapeutics, Glycosylation, Dr. Stefana Petrescu (Ed.), ISBN: 978-953-51-0771-2, InTech, DOI: 10.5772/50261.

Sou SN, Ilieva KM, Polizzi KM (2012). Binding of human BiP to the ER stress transducers IRE1 and PERK requires ATP. Biochem Biophys Res Commun. 420(2):473-8.

Contact


Phone No: 0131 650 5416

Email: si.sou@ed.ac.uk

Links


LinkedIn: https://uk.linkedin.com/in/si-nga-sou-36181439