Dr Maryia Trubitsyna

 

Maryia Trubitsyna

 
Background

Maryia completed her undergraduate studies in Belarussian State University, Minsk, where she contributed to production of veterinary pharmaceutical product based on interferon. For her PhD she came to Edinburgh University and joined Prof David Finnegan's group, where she studied molecular mechanisms of transposition in collaboration with Dr Julia Richardson. After completion of her PhD she joined the Flowers Consortium as a postdoc working with Prof Christopher French. During these two years Maryia has developed a new DNA assembly method - PaperClip.
 
Research

Maryia is a lab manager and a postdoctoral research assosiate. She is currently working on developing alternative DNA-dependent RNA polymerases to improve the efficiency of in vitro transcription. The main goal is to obtain higher yeild and quality of RNA for various applications. 
 
Publications

Roy Choudhury, N., Heikel, G., Trubitsyna, M., Kubik, P., Nowak, J. S., Webb, S., Granneman, S., Spanos, C., Rappsilber, J., Castello, A., Michlewski, G. RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. BMC Biology, 15:105 doi:10.1186/s12915-017-0444-9 (2017).

Trubitsyna, M.*, Michlewski, G., Finnegan, D. J., Elfick, A., Rosser, S. J., Richardson, J. M., & French, C. E. Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection. Nucleic Acids Research, 45(10), e89-e89. doi:10.1093/nar/gkx113 (2017).
* co-corresponding author

Trubitsyna, M.*, Liu, C.K., Salinas, A., Elfick, A. and French, C.E. PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly. Methods Mol Biol, 1472, 111-128, doi:10.1007/978-1-4939-6343-0_9 (2017).
* co-corresponding author

Trubitsyna, M., Grey, H., Houston, D. R., Finnegan, D. J. & Richardson, J. M. Structural Basis for the Inverted Repeat Preferences of mariner Transposases. The Journal of Biological Chemistry, doi:10.1074/jbc.M115.636704 (2015).

Trubitsyna, M., Chan, K., Cai, Y., Elfick, A., French, C., BBF RFC 104: BrickClip - rapid assembly of multiple RFC10 BioBricks, doi:1721.1/96070 (2015).

Trubitsyna, M.*, Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res 42, e154, doi:10.1093/nar/gku829 (2014).
* co-corresponding author

Trubitsyna, M., Morris, E. R., Finnegan, D. J. & Richardson, J. M. Biochemical characterization and comparison of two closely related active mariner transposases. Biochemistry 53, 682-689, doi:10.1021/bi401193w (2014).

Wolkowicz, U. M., Morris, E. R., Robson, M., Trubitsyna, M. & Richardson, J. M. Structural basis of Mos1 transposase inhibition by the anti-retroviral drug Raltegravir. ACS Chemical Biology 9, 743-751, doi:10.1021/cb400791u (2014).

Gasiunaite, L., Lewicka, A., Pashkuleva, H., Villanueva, H., Thornton, H., Trubitsyna, M. and French, C., BBF RFC 98: Genbrick - A Rapid Multi-part Assembly Method for BioBricks, doi:1721.1/81331 (2013).

Cuypers, M. G., Trubitsyna, M., Callow, P., Forsyth, V. T. & Richardson, J. M. Solution conformations of early intermediates in Mos1 transposition. Nucleic Acids Res 41, 2020-2033, doi:10.1093/nar/gks1295 (2013).

 
Contact

Phone No: 0131 650 54 16